Part:BBa_K4343098

PaDes17

PaDes17 encodes ∆-17 desaturase from Pythium aphanidermatum which functions in the Δ-9 elongase/desaturase pathway and could convert C20:4 to C20:5.

Description

The PaDes17 gene encodes ∆-17 desaturase of 359 amino acids from Pythium aphanidermatum that catalyzes the conversion of arachidonic acid (ARA; C20:4 n-6) to Eicosapentaenoic acid (EPA; C20:5 n-3).

Literature

The results showed that the ∆-17 desaturase gene was expressed in the arachidonic acid producing strain of Y. lipolytica.. It converts -6 arachidonic acid to -3 eicosapentaic acid with substrate conversion efficiencies of 54 to 65%.

Reference

Xue, Z., He, H., Hollerbach, D., Macool, D. J., Yadav, N. S., Zhang, H., ... & Zhu, Q. (2013). Identification and characterization of new Δ-17 fatty acid desaturases. Applied microbiology and biotechnology, 97(5), 1973-1985.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 433
  Illegal XhoI site found at 175
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1
  Illegal BsaI.rc site found at 1097